Data for: Honey bee beards: internal and external factors driving mass thermoregulatory evacuation

This dataset contains the following files

(1) An .R file containing the R code used to run the statistical analysis and produce the figures.

(2) The file 'dailypattern_colonysize.xlsx' contains two sheets : 'beard_dailypattern_colonysize' and 'temp_dailypattern_colonysize' which contains the colony bearding information and the externaltemperature respectively. This data is used to answer 2 research investigations: 1) How does beard size change throughout the day? (2) How does number of adult workers in the colony (colony state) impact bearding?
 - 'beard_dailypattern_colonysize.xlsx' have 12 elements:
 date_time - the date and time stamp for each observation
 colony - the individual id assigned to a colony
 site - "grass" or "concrete" (the name of the apiary where the colony was housed during the observation period )
 end_colony_size - colony size estimate from the the Liebefeld method at the end of the observation period
 hour - the hour window of the observation
 Exp_day - the experiment day since the start of the observation (6/7/2023 12PM- 6/8/2023 11:59AM is the Day 1 of experiment)
 bee_count_raw - the estimated number of the bees at the entrance of the colony
 bee_count - the estimated number of bees in the beard from the volume and loose count (cut-off 25 bees, if bee_count_raw less than 25 then bee_count=0)
 per_south - proportion of the beard in the south side (number of bees in south/bee_count)
 per_east - proportion of the beard in the east side (number of bees in east/bee_count)
 per_west - proportion of the beard in the west side (number of bees in west/bee_count)
 scaled_count - it is scaled beard size calculated by dividing the number of bees in the beard (bee_count) at each timepoint by the biggest beard for that colony in an experiment day (Exp_day)
 - 'temp_dailypattern_colonysize' has 3 elements
 date - the date stamp for temperature reading
 hour - the hour stamp for temperature reading
 temp - the ambient/external temperature value in degree celsius.
 
 (3) The file 'broodstatus.xlsx' contains two sheets: 'beard_broodstatus' and 'temp_broodstatus' which contains the colony beard information and the within hive temperature respectively. This data is used to answer the research investigation: (2) How does presence of brood in the colony (colony state) impact bearding?
 -'beard_broodstatus' have 7 elements:
 date_time - the date and time stamp for each observation
 colony - the individual id assigned to the colony
 round - "1" or "2" (to specify the round of the observation: Round 1: 08/10/2023 12PM-08/13/2023 6AM; Round 2: 08/15/2023 12PM-08/18/2023 6AM)
 group - "Brood" or "No-Brood" (the brood status of the colony)
 Exp_day - the experiment day since the start of the observation (08/10/2023 12PM- 08/11/2023 11:59AM is the Day 1 of experiment)
 bee_count_raw - the estimated number of the bees at the entrance of the colony
 bee_count - the estimated number of bees in the beard from the volume and loose count (cut-off 25 bees, if bee_count_raw less than 25 then bee_count=0)
 -'temp_broodstatus' have 6 elements:
 date - the date stamp for the temperature reading
 hour - the hour stamp for temperature reading
 colony - the individual id assigned to the colony
 group - "Brood" or "No-Brood" (the brood status of the colony)
 round - "1" or "2" (to specify the round of the observation: Round 1: 08/10/2023 12PM-08/13/2023 6AM; Round 2: 08/15/2023 12PM-08/18/2023 6AM)
 Temperature -the within hive temperature value in degree celsius.
 
 (4) The file 'timeofday.xlsx' contains two sheets: 'beard_timeofday' and 'temp_timeofday' which contains the colony beard information and the within hive brood temperature respectively. This data is used to answer the research investigation: (3) How time of day influences beard formation and dissipation during a heat stress? 
 -'beard_timeofday' have 8 elements:
 date_time - the date and time stamp for each observation
 hs_start - the date and time stamp for start of the heat stress
 colony -  the individual id assigned to the colony
 trial - "1" or "2" to specify the first trial and the second trial when the colony was heat stressed
 group - "Night" vs "Day" (the time when the colony was heat stressed - day: around 2-3PM; Night: around 7-7:30PM)
 area - the area estimate of the beard from the photo
 loose_bees - the number of bees on the entrance which are not included in the area estimate/are just counted
 relative_time - the time (in hrs) since the start of the heat stress
 -'temp_timeofday' have 6 elements:
 date_time - the date and time stamp for each temperature reading
 colony -  the individual id assigned to the colony
 group - "Night" vs "Day" (the time when the colony was heat stressed - day: around 2-3PM; Night: around 7-7:30PM)
 temp - the within hive brood temperature value in degree celsius.
 hs_start - the date and time stamp for start of the heat stress
 relative_time - the time (in minutes) since the start of the heat stress
 
 (5) The file 'illumination.xlsx' contains one sheet: 'beard_illumination' which contains the colony beard information. This data is used to answer the research investigation: (4) The impact of light on beard dissipation
 -'beard_illumination' have 9 elements:
 date_time - the date and time stamp for each observation
 colony -  the individual id assigned to the colony
 group - "Control" or "Treatment" (Control nights: dark nights or no illumination; Treatment nights: artifically illunminated from 2300-0100)
 hour - the hour window of the observation
 minute - the minute window of the observation
 Exp_day - the experiment day since the start of the observation (8/18/2023 6PM- 8/19/2023 5:59PM is the Day 1 of experiment)
 bee_count_raw - the estimated number of the bees at the entrance of the colony
 bee_count - the estimated number of bees in the beard from the volume and loose count (cut-off 25 bees, if bee_count_raw less than 25 then bee_count=0)
 scaled_count - it is scaled beard size calculated by dividing the number of bees in the beard (bee_count) at each timepoint by the biggest beard for that colony in an experiment day (Exp_day)