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Altered microRNA Expression Profiles and Regulation of INK4A/CDKN2A Tumor Suppressor Genes in Canine Breast Cancer Models


Metadata FieldValueLanguage
dc.contributorRichard Curtis Bird, birdric@auburn.eduen_US
dc.creatorMohammad, Farruk
dc.creatorKabir, Lutful
dc.creatorDelnnocentes, Patricia
dc.creatorBird, Richard Curtis
dc.date.accessioned2020-01-16T17:19:48Z
dc.date.available2020-01-16T17:19:48Z
dc.date.created2015
dc.identifier10.1002/jcb.25243en_US
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/pdf/10.1002/jcb.25243en_US
dc.identifier.urihttp://hdl.handle.net/11200/49686
dc.description.abstractmicroRNA (miRNA) expression profiling of cancer versus normal cells may reveal the characteristic regulatory features that can be correlated to altered gene expression in both human and animal models of cancers. In this study, the comprehensive expression profiles of the 277 highly characterized miRNAs from the canine genome were evaluated in spontaneous canine mammary tumor (CMT) models harboring defects in a group of cell cycle regulatory and potent tumor suppressor genes of INK4/CDKN2 family including p16/INK4A, p14ARF, and p15/INK4B. A large number of differentially expressed miRNAs were identified in three CMT cell lines to potentially target oncogenes, tumor suppressor genes and cancer biomarkers. A group of the altered miRNAs were identified by miRNA target prediction tools for regulation of the INK4/ CDKN2 family tumor suppressor genes. miRNA-141 was experimentally validated for INK4A 30 -UTR target binding in the CMT cell lines providing an essential mechanism for the post-transcriptional regulation of the INK4A tumor suppressor gene in CMT models. A wellrecognized group of miRNAs including miR-21, miR-155, miR-9, miR-34a, miR-143/145, and miR-31 were found to be altered in both CMTs and human breast cancer. These altered miRNAs might serve as potential targets for advancing the development of future therapeutic reagents. These findings further strengthen the validity and use of canine breast cancers as appropriate models for the study of human breast cancers.en_US
dc.formatPDFen_US
dc.relation.ispartofJournal of Biological Chemistryen_US
dc.relation.ispartofseries0021-9258en_US
dc.subjectmiRNA; BREAST CANCER; TUMOR SUPPRESSOR; CYCLIN-DEPENDENT KINASE INHIBITOR; INK4A; CANINE CANCER MODELen_US
dc.titleAltered microRNA Expression Profiles and Regulation of INK4A/CDKN2A Tumor Suppressor Genes in Canine Breast Cancer Modelsen_US
dc.typeTexten_US
dc.type.genreJournal Article, Academic Journalen_US
dc.citation.volume116en_US
dc.citation.issue12en_US
dc.citation.spage2956en_US
dc.citation.epage2969en_US
dc.description.statusPublisheden_US
dc.description.peerreviewYesen_US

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