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Development of endocytosis, degradative activity, and antigen processing capacity during GM-CSF driven differentiation of murine bone marrow


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dc.contributorElizabeth Schwartz, ehs0009@auburn.eduen_US
dc.creatorOlatunde, Adesola C.
dc.creatorAbell, Laura P.
dc.creatorLanduyt, Ashley E.
dc.creatorSchwartz, Elizabeth Hiltbold
dc.date.accessioned2022-03-11T13:54:40Z
dc.date.available2022-03-11T13:54:40Z
dc.date.created2018-05-10
dc.identifier10.1371/journal.pone.0196591en_US
dc.identifier.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0196591en_US
dc.identifier.urihttps://aurora.auburn.edu/handle/11200/50047
dc.identifier.urihttp://dx.doi.org/10.35099/aurora-116
dc.description.abstractDendritic cells (DC) are sentinels of the immune system, alerting and enlisting T cells to clear pathogenic threats. As such, numerous studies have demonstrated their effective uptake and proteolytic activities coupled with antigen processing and presentation functions. Yet, less is known about how these cellular mechanisms change and develop as myeloid cells progress from progenitor cells to more differentiated cell types such as DC. Thus, our study comparatively examined these functions at different stages of myeloid cell development driven by the GM-CSF. To measure these activities at different stages of development, GM-CSF driven bone marrow cells were sorted based on expression of Ly6C, CD115, and CD11c. This strategy enables isolation of cells representing five distinct myeloid cell types: Common Myeloid Progenitor (CMP), Granulocyte/Macrophage Progenitor (GMP), monocytes, monocyte-derived Macrophage/monocyte-derived Dendritic cell Precursors (moMac/moDP), and monocyte-derived DC (moDC). We observed significant differences in the uptake capacity, proteolysis, and antigen processing and presentation functions between these myeloid cell populations. CMP showed minimal uptake capacity with no detectable antigen processing and presenting function. The GMP population showed higher uptake capacity, modest proteolytic activity, and little T cell stimulatory function. In the monocyte population, the uptake capacity reached its peak, yet this cell type had minimal antigen processing and presentation function. Finally, moMac/moDP and moDC had a modestly decreased uptake capacity, high degradative capacity and strong antigen processing and presentation functions. These insights into when antigen processing and presentation function develop in myeloid cells during GM-CSF driven differentiation are crucial to the development of vaccines, allowing targeting of the most qualified cells as an ideal vaccine vehicles.en_US
dc.formatPDFen_US
dc.publisherPUBLIC LIBRARY of SCIENCEen_US
dc.relation.ispartofPLoS Oneen_US
dc.relation.ispartofseries1932-6203en_US
dc.rightsThis is the version of record published by PLoS and is made available under the CC-BY 4.0 license. Item should be cited as: Olatunde, A. C., Abell, L. P., Landuyt, A. E., & Hiltbold Schwartz, E. (2018). Development of endocytosis, degradative activity, and antigen processing capacity during GM-CSF driven differentiation of murine bone marrow. PLoS One, 13(5), e0196591.en_US
dc.subjectDENDRITIC CELL-DEVELOPMENTen_US
dc.subjectCROSS-PRESENTATIONen_US
dc.subjectSTEADY-STATEen_US
dc.subjectFLT3 LIGANDen_US
dc.subjectMACROPHAGESen_US
dc.subjectRECEPTORen_US
dc.subjectPHAGOCYTOSISen_US
dc.subjectPROGENITORSen_US
dc.subjectINFECTIONen_US
dc.titleDevelopment of endocytosis, degradative activity, and antigen processing capacity during GM-CSF driven differentiation of murine bone marrowen_US
dc.typeTexten_US
dc.type.genreJournal Article, Academic Journalen_US
dc.citation.volume13en_US
dc.citation.issue5en_US
dc.citation.spagee0196591en_US
dc.description.statusPublisheden_US
dc.description.peerreviewYesen_US

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